Select the correct statement from the following :

Biology Biotechnology Principles and Processes Previous Year Questions Single Correct MCQ

Published on: August 12, 2026

Gel electrophoresis is used for amplification of a DNA segment.

The polymerase enzyme joins the gene of interest and the vector DNA.

Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.

PCR is used for isolation and separation of gene of interest.

Text Solution

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The correct answer is:

Restriction enzymes cut DNA at specific sites under suitable conditions (temperature, buffer, pH). The other statements are incorrect because:

• Gel electrophoresis separates DNA, not amplifies it

• DNA ligase (not polymerase) joins gene and vector

• PCR amplifies DNA, not isolates/separates genes

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